PNA, antisense and antimicrobials
نویسندگان
چکیده
منابع مشابه
Peptide nucleic acid (PNA) antisense effects in Escherichia coli.
Antisense peptide nucleic acid (PNA) can be used to control cell growth, gene expression and growth phenotypes in the bacteria Escherichia coli. PNAs targeted to the RNA components of the ribosome can inhibit translation and cell growth, and PNAs targeted to mRNA can limit gene expression with gene and sequence specificity. In an E. coli cell extract, efficient inhibition is observed when using...
متن کاملCPP or Cholesterol Conjugated Antisense PNA for Cellular Delivery
Peptide nucleic acid (PNA) is a DNA mimic consisting of the four common bases of DNA on a pseudopeptide backbone that makes it extremely stable in biological fluids. Antisense PNA is targeted against mRNA in cytoplasm in a sequence specific manner. However, the main hindrance to the effective use of PNAs has been their relatively poor uptake by cells. Endosomal release or direct uptake into cyt...
متن کاملA highly effective and long-lasting inhibition of miRNAs with PNA-based antisense oligonucleotides.
MiRNAs are non-coding RNAs that play a role in the regulation of major processes. The inhibition of miRNAs using antisense oligonucleotides (ASOs) is a unique and effective technique for the characterization and subsequent therapeutic targeting of miRNA function. Recent advances in ASO chemistry have been used to increase both the resistance to nucleases and the target affinity and specificity ...
متن کاملExpanding the repertoire of pyrrolidyl PNA analogues for DNA/RNA hybridization selectivity: aminoethylpyrrolidinone PNA (aepone-PNA).
New PNA analogues derived from aminoethylpyrrolidin-5-one backbone show stabilization of aepone-PNA:DNA hybrids and destabilization of the corresponding RNA hybrids compared to unmodified PNA.
متن کاملPNA Chips
Definition Peptide nucleic acid (PNA) is an artificial mimic of nucleic acid, which was invented more than a decade ago (7). The molecule combines many features that are similar to those of natural nucleic acids with characteristics that are rather different or even unique to PNA. First and foremost, N-(2-aminoethyl)-glycine units linked by ▶amide bonds substitute the (deoxy)ribosephosphate bac...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 1998
ISSN: 1087-0156,1546-1696
DOI: 10.1038/nbt0498-332